Lipid accumulation, lipid oxidation, and low plasma levels of. Several lipidregulating loci in the mouse population harbor genes associated with abnormal lipid metabolism in human gwas. Sep 24, 2019 understanding genetic architecture of plasma lipidome could provide better insights into lipid metabolism and its link to cardiovascular diseases cvds. The plasma lipidome in acute myeloid leukemia at diagnosis in. Stressresistant lipidomic signature confers extreme. Genetic regulation of plasma lipid species and their. Human plasma proteome project data central at peptideatlas. Identification of additional lipid species in human plasma. Lipidomics of human blood plasma by highresolution shotgun. Although blood lipid metabolites are considered to be potential biomarker candidates, their fundamental properties are not well characterized.
In this study, we used metabolomics and lipidomics approaches paired with univariate and multivariate statistical analyses to assess the effects of anticoagulant, temperature, and time on healthy human plasma samples collected to provide guidelines on sample collection, handling, and processing for vaccinology. Pdf human blood is a selfregenerating, lipidrich biologic fluid that is routinely. The amount of plasma antibodies bound was measured with alkaline phosphatase labeled antihumanigg and antihumanigm antibodies sigmaaldrich. Download fulltext pdf genetic architecture of human plasma lipidome and its link to cardiovascular disease article pdf available in nature communications 101. Download acrobat pdf file 1mb transparency document. Machine learning of human plasma lipidomes for obesity estimation. Large scale lipid profiling of a human serum lipidome using a.
Merge and align search results from all 24 raw files for lipid profiling. The methodology allowed for the clustering of samples by disease state when data is analyzed using multivariate statistical packages. Mar 19, 2019 effects of aged stored autologous red blood cells on human plasma metabolome. We aimed to 1 investigate the matrix type serum vs. L of plasma, using a new commercially available readytospikein mixture with 14 synthetic lipid standards, and executing a single 6 min sample injection with combined msx analysis for cholesterol. The method was shown to be linear over four orders of magnitude and had sufficient sensitivity to allow for the analysis of lipids at endogenous levels in human plasma. Quantitative analysis of these and closely related lipids in human plasma from two cohorts reveal that dihydroceramides are significantly elevated in individuals progressing to diabetes, up to 9 years before disease onset.
Rantamaki, tuulikki seppanenlaakso, matej oresic, matti jauhiainen, juha m. Oct 23, 2017 to further show the efficacy of this approach, we devised a simple routine for extensive plasma lipidome characterization using only 8. Aims we tested whether characteristic changes of the plasma lipidome in individuals with comparable total lipids level associate with future cardiovascular disease cvd outcome and whether 23 validated gene variants associated with coronary artery disease cad affect cvd associated lipid species. Blood plasma lipidome profile of dairy cows during the. Biomolecules free fulltext the effect of anticoagulants. We therefore speculate that the starting phase of plasma lipidomics is over. Omenn internal medicine, human genetics, public health and center for computational medicine and biology, university of michigan, ann arbor, mi, usa the human proteomics organization hupo human plasma proteome project ppp is a.
Plasma proteins albumins 65%, osmolarity and viscosity globulins transport and storage proteins transferrin carries iron in blood ferritin stores iron in liver and marrow globulins antibodies fibrinogens clotting proteins. Plasma dihydroceramides are diabetes susceptibility biomarker. The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from. Genetic investigation of human plasma lipidome to get insights into lipidrelated disorders beyond. We aimed to 1 determine heritability of lipid species and their genetic correlations. A longitudinal plasma lipidomics dataset from children who. Considering that the human plasma lipidome contains at least 600 named and quantifiable lipid species 45 but probably many more 46, our assays captured only a small part of the possible. Human blood is a selfregenerating lipidrich biological fluid that is routinely collected in hospital. Genetic correlation of the plasma lipidome with type 2. The lipid composition of human skin is essential for its function. Identification of key lipids critical for platelet activation.
The effect of anticoagulants, temperature, and time on the human. Plasma lipidome reveals critical illness and recovery from human. The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from healthy donors as determined by. Oct 28, 20 antigens were immobilized on white 96well microtiter plates at 5.
Human plasma for the purpose of metabolite analysis. The results highlight the profound impact of evd on the host lipidome, leading to possible therapies for improving evd survival. The first cell lipidome to be published was that of a mouse macrophage in 2010. The human plasma metabolome is composed of around 4,229 confirmed compounds that can be grouped into more than 50 chemical classes. Apr 10, 2018 it is unknown if the plasma lipidome is a useful tool for improving our understanding of the acute respiratory distress syndrome ards. Here we describe a method for the absolute molar quantification of more than 200 molecules from 14 major lipid classes from 5. Genetic architecture of human plasma lipidome and its. The plasma lipid profile therefore also represents a lifestyle modifiable factor which can play a decisive role in the health state and maintenance of cognitive function during aging. The human proteome organization in 2003 launched an effort to combine results from the many labs around the world who were working on the human plasma proteome. Methods and results we performed a genomewide association study gwas of 141 lipid species n2,181 individuals, followed by phenomewide scans phewas with 44 clinical endpoints related to cardiometabolic, psychiatric and gastrointestinal disorders n456,941. Using liquid chromatography tandem mass spectrometry, we profiled the human plasma lipidome of patients that survived and succumbed to evd during the 2014 outbreak in sierra leone. Plasma and serum lipidomics of healthy white adults shows. This effort, the human plasma proteome project, continues today and the peptideatlas is an integral part of that effort.
Whether the wide interindividual variability in the plasma lipidome contributes to the genetic basis of t2d is unknown. Aug 21, 2019 consequently, the plasma lipidome has a considerable impact on the cellular lipid environment, vasculature function, and inflammatory and oxidative processes. Because of its technical simplicity and robustness, the protocol lends itself for highthroughput clinical lipidomics screens. Human blood is a selfregenerating lipidrich biological fluid that is routinely collected in hospital settings. The hppp was initiated in 2002 as the means to overcome the major challenges e. For shotgun lipidomics, this can be obtained in a single mass spectrometric measurement after direct infusion of the sample. Internal medicine is in flux because of the omics revolution, with cancer medicine being a good example. Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome. The lipidome of the yeast saccharomyces cerevisiae has been characterised with an estimated 95% coverage. Pdf the effect of anticoagulants, temperature, and time. Human plasma lipidome has been extensively studied in many pathophysiological contexts with the hope of identifying biomarkers for early diagnostics and monitoring the progression and treatment of a broad spectrum of diseases. Understanding perturbations in the aml plasma lipidome may lead to new targets for drug therapy of aml.
Human plasma comprises nucleic acids, amino acids mostly in the form of proteins, carbohydrates in the form of monosaccharides and disaccharides, and lipids fig. The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from healthy donors as determined by liquid chromatographymass spectrometry. Lipidomics reveals a remarkable diversity of lipids in human. Breaking up prolonged sitting alters the postprandial plasma. Among these classes, lipids including glycerolipids, phospholipids, fatty acids, glycolipids, and sphingolipids represent more than 80% of the human plasma metabolome. We hypothesized that interrupting sitting time would differentially alter postprandial plasma lipidome profiles, in comparison with sit. Blood is a commonly used biofluid for biomarker discovery. Gerspach 0 0 department of farm animals, vetsuisse faculty, university of zurich, winterthurerstrasse 260, 8057 zurich, switzerland 1 institute of pharmacology and toxicology, vetsuisse faculty, university of zurich. Here, we investigated the potential overlap in the genetic basis of the plasma lipidome and t2drelated traits.
The inventory of lipid molecules found in blood plasma plasma lipidome offers insights into individual metabolism and physiology in health and disease. For example, the human plasma lipidome consist of almost 600 distinct molecular species. The effect of anticoagulants, temperature, and time on the. Quantifying the lipidome of transgenic mice tissue. Global lipidome profile of evd human plasma per lipid subclass. Most of the proteins found in blood plasma are manufactured in the liver. Lipidomics is the largescale study of pathways and networks of cellular lipids in biological systems the word lipidome is used to describe the complete lipid profile within a cell, tissue, organism, or ecosystem and is a subset of the metabolome which also includes the three other major classes of biological molecules. Pdf msbased lipidomics of human blood plasma a community. Plasma lipidome reveals critical illness and recovery from. Of all lipids determined in this study, plasma pgf2.
The human plasma proteome holds the promise of a revolution in disease diagnosis and therapeutic monitoring provided that major challenges in proteomics and related disciplines can be addressed. Biochemical analyses reveal that plasma contains over 600 structural forms of lipid molecules. Genetic architecture of human plasma lipidome and its link. The data presented in the figures and other summary level data are contained within the supplementary files and. Genetic architecture of human plasma lipidome and its link to. Pdf genetic architecture of human plasma lipidome and.
Molecular technologies that detect alterations in genebased structure or function are having an impact on diagnosis, prognosis and treatment of cancer. Bmc veterinary research blood plasma lipidome profile of dairy cows during the transition period s. Therefore, we measured the plasma lipidome of individuals with ards at two timepoints to determine if changes in the plasma lipidome distinguished survivors from nonsurvivors. From composition to function plos one, may 2011 antti h. Aim genetic investigation of human plasma lipidome to get insights into lipidrelated disorders beyond traditional lipid measures. May 19, 2017 differential plasma concentrations of circulating lipid species are associated with pathogenesis of type 2 diabetes t2d. Most plasma metabolites and lipids were unaffected by storage time and temperature. Aug 02, 2018 bing peng, sascha geue, cristina coman, patrick munzer, dominik kopczynski, canan has, nils hoffmann, mailinchristin manke, florian lang, albert sickmann, meinrad gawaz, oliver borst, robert ahrends. Msbased lipidomics of human blood plasma a communityinitiated position paper to develop accepted guidelines. L of human blood plasma using highresolution topdown shotgun mass spectrometry. This trial was approved by the alfred hospital human ethics committee melbourne, vic 3004, australia, and was carried out in accordance with the declaration of helsinki. Methods and results screening of the fasted plasma lipidome was performed by topdown shotgun. Nov 10, 2011 human plasma comprises nucleic acids, amino acids mostly in the form of proteins, carbohydrates in the form of monosaccharides and disaccharides, and lipids fig. Associations of the plasma lipidome with mortality in the.
We report here for the first time an indepth lipid profile of human plasma that reveals the enormous structural diversity of lipids comprising the six major lipid categories as defined by lipid maps. Nov, 2018 a total of 428 plasma samples were extracted in randomised order using a modified version of the folch procedure. The hppp provided the strategy to deal with the growing numbers of plasma samples being systematically collected, stored, and accessible through biobanks. The effect of anticoagulants, temperature, and time on the human plasma metabolome and lipidome from healthy.